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Mutations conferring GSH-independence map to the protomer interface between proteins VP1 and VP3 and are consistent with GSH binding being essential for the formation of pentameric subunits critical to assembly 24,27

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Gingerols and shogaols, its active constituents, enhance gastric motility, accelerate gastric emptying, and reduce visceral hypersensitivity

To test this data interpretation, we analyzed the GSSG-dependent oxidation of reduced fusion constructs between roGFP2 WT and wild-type PfGrx (PfGrx WT ), the dithiol mutant PfGrx C88S , or the monothiol mutant PfGrx C32S/C88S (Fig